GR (Phospho-Ser226) Antibody

Description

Product Name GR (Phospho-Ser226) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of GR only when phosphorylated at serine 226.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 226 (L-L-S(p)-P-L) derived from Human GR.
Target Name GR
Modification Phospho-Ser226
Alternative Names GCCR; GCR; GRL; NR3C1;
Accession No. Swiss-Prot#: P04150;
NCBI Gene#: 2908;
NCBI Protein#: NP_000167.1
SDS-PAGE MW 86kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) antibody #AB11937.The lane on the right is treated with the antigen-specific peptide.

Background

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.

Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) J Biol Chem 289, 10235-51
Bouazza B, et al. (2014) Am J Respir Cell Mol Biol 50, 301-15
Lee MS, et al. (2013) Behav Brain Res 236, 56-61

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