CDK5 (Phospho-Tyr15) Antibody

Description

Product Name CDK5 (Phospho-Tyr15) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of CDK5 only when phosphorylated at tyrosine 15.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Tyrosine15 (G-T-Y(p)-G-T) derived from Human CDK5.
Target Name CDK5
Modification Phospho-Tyr15
Alternative Names CDK5; CRK6; Cell division protein kinase 5; Tau protein kinase II catalytic subunit; kinase Cdk5
Accession No. Swiss-Prot#: Q00535;
NCBI Gene#: 1020;
NCBI Protein#: NP_001157882.1
SDS-PAGE MW 33kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from COS7 cells treated with EGF using Phospho-CDK5 (Tyr15) antibody #AB11918.The lane on the right is treated with the antigen-specific peptide.

Background

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM.

Miao Y, et al. (2012)PLoS One 7, e42318. Li P, et al. (2011)Cell 147, 815-26. Nohara A, et al. (2011)J Biol Chem 286, 33457-65.

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