Ezrin (Phospho-Tyr146) Antibody

Description

Product Name Ezrin (Phospho-Tyr146) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 146.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 146 (S-G-Y(p)-L-S) derived from Human Ezrin.
Target Name Ezrin
Modification Phospho-Tyr146
Alternative Names Cytovillin; EZRI; p81; VIL2; Villin 2
Accession No. Swiss-Prot#: P15311;
NCBI Gene#: 7430;
NCBI Protein#: NP_001104547.1
SDS-PAGE MW 69kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from 293 cells treated with UV using Phospho-Ezrin (Tyr146) antibody #AB11909.The lane on the right is treated with the antigen-specific peptide.

Background

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Murchie R, et al. (2014)Proc Natl Acad Sci U S A 111, 693-8. Kawahara T, et al. (2013)PLoS One 8, e79654 . Wang Y, Lazo JS (2012)Oncogene 31, 818-27.

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