PDGFRb (Phospho-Tyr771) Antibody

Description

Product Name PDGFRb (Phospho-Tyr771) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of PDGFRb only when phosphorylated at tyrosine 771.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine 771 (S-N-Y(p)-M-A) derived from Human PDGFRb.
Target Name PDGFRb
Modification Phospho-Tyr771
Alternative Names CD140b; PDGF-R-beta; PDGFR; PGFRB; kinase PDGFR-beta
Accession No. Swiss-Prot#: P09619;
NCBI Gene#: 5159;
NCBI Protein#: NP_002600.1
SDS-PAGE MW 190kd
Concentration 1.0mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HepG2 tissue using PDGFRb (Phospho-Tyr771) antibody #AB11907.The lane on the right is treated with the antigen-specific peptide.

Background

PDGF Receptor β encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Wardega P, Heldin CH, Lennartsson J (2010)Cell Signal 22, 1363-8. Persson C, et al. (2004) Mol Cell Biol 24, 2190-201. Ekman S, et al. (2002)Oncogene 21, 1870-5.

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