EGFP/EYFP Monoclonal Antibody

Description

Product Name EGFP/EYFP Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Clone No. 3F11-B12-E7-F12
Isotype IgG1
Purification Affinity purified
Applications WB
Species Reactivity Bacillus cereus
Specificity Transfected
Immunogen Type Recombinant Protein
Immunogen Description Purified recombinant EYFP.tag full length expressed in E.coli.
Target Name EGFP/EYFP
Alternative Names GFP; Green Fluorescent Protein; enhanced Green Fluorescent Protein;
Accession No. Uniprot: C8CHS1
SDS-PAGE MW According
Formulation Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage store at -20˚C

Application Details

Western blotting: 1:1000

Images

Western blot detection of EGFP expression in 293T cells transfected (A:mRFP) or transfected (B:EGFP fused protein;C:EGFP) using EGFP antibody(L:1/60000 diluted. M:1/20000 diluted. R:1/10000 diluted).
Western blot detection of EYFP expression in Rosetta E.coli cells induced by 0(A)or 0.1mM (B)IPTG using EGFP antibody(1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.
Western blot detection of EGFP expression in Hela cells non-transfected (A) or transfected (B) with pEGFP C1 using EGFP antibody(1:5000 diluted).Predicted band size: 34KDa Observed band size: 34KDa.

Background

The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

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