HP1-gamma Monoclonal Antibody

Description

Product Name HP1-gamma Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Clone No. 5G10-F7-A12
Isotype IgG1
Purification Affinity purified
Applications WB ICC IP IHC
Species Reactivity Ms Hu Mk Hm Rt
Specificity This antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.
Immunogen Type Recombinant Protein
Immunogen Description Purified recombinant human HP1-gamma protein fragments expressed in E.coli.
Target Name HP1-gamma
Alternative Names CBX 3; CBX3; CBX3_HUMAN; Chromobox homolog 3 (HP1 gamma homolog, Drosophila); Chromobox homolog 3; Chromobox protein homolog 3; GAMMA; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma
Accession No. Uniprot: Q13185
Gene ID: 11335
SDS-PAGE MW 22kd
Formulation Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage store at -20˚C

Application Details

Western blotting: 1:1000

Immunocytochemistry: 1:200

Images

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma antibody (dilution 1:200).
Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma antibody (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.
Immunoprecipitation analysis of Hela cell lysates using HP1-gamma antibody.
Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma Mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Background

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Published Papers

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