Mouse anti-Human CD19, Purified mAb

Description

Product Name Mouse anti-Human CD19, Purified mAb
Host Species Mouse
Clonality Monoclonal
Clone No. COC19
Isotype IgG1
Applications FC
Species Reactivity Hu
Target Name CD19
Concentration 1mg/ml
Formulation Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
Storage Store at 4°C. DO NOT FREEZE.

Application Details

Notice: This reagent has been pre-diluted for use at recommended volume per test in flow cytometry analysis. Typically add 10ul of this reagent to 100μl of experimental sample with 1 X 106 cells per test. Please refer to the detailed protocol when you perform a test.

Images

Human peripheral blood lymphocytes analyzed with Purified CD19 mAb, followed by anti-mouse IgGs-PE

Background

COC19 reacts with CD19 (B4), a 90 kDa molecule, which is expressed on approximately 5-25% of human peripheral blood lymphocytes. CD19 antigen is present on human B lymphocytes at most stages of maturation, from the earliest Ig gene rearrangement in pro-B cells to mature cell, as well as malignant B cells, but is lost on maturation to plasma cells. CD19 antibody does not react with T lymphocytes, monocytes and granulocytes. CD19 is a critical signal transduction molecule that regulates B lymphocyte development, activation and differentiation. This clone is cross reactive with non-human primate.

* CD19 is a key phenotyping marker of non-T cell leukemia.

1. Nadler, LM et al. (1983) J. Immunol. 131:244

2. Schlossman, SL et al., eds. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York.

3. Tedder T. et al. (1994) Immunol Today. 15:437-442

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