PAK1/PAK2/PAK3(Ab-423/402/421) Antibody

Description

Product Name PAK1/PAK2/PAK3(Ab-423/402/421) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.421~425/400~404/419~423 (R-S-T-M-V) derived from Human PAK1/PAK2/PAK3.
Target Name PAK1/PAK2/PAK3
Alternative Names p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;
Accession No. Swiss-Prot: Q13153/Q13177/O75914
NCBI Protein: NP_001122092.1 NP_002568.2 NP_001121638.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 61-67 (PAK2) 68-74 (PAK1/3)kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from MCF-7 and Hela cells using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #AB21169.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #AB21169(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Ab-423/402/421) Antibody #AB21169.

Background

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

Published Papers

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