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Mouse anti-Human CD3, Purified mAbAB28003

  Catalog No. Package Size Price
AB28003 100 ug $279.00

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Description
  • Product Name Mouse anti-Human CD3, Purified mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Clone No. 4AT3
  • Isotype Mouse IgG2a
  • Applications FC
  • Species Reactivity Hu
  • Target Name CD3
  • Concentration 1mg/ml
  • Formulation Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
  • Storage Store at 4°C. DO NOT FREEZE.
Application Details

 Notice: This reagent has been pre-diluted for use at recommended volume per test in flow cytometry analysis. Typically add 10ul of this reagent to 100μl of experimental sample with 1 X 106 cells per test. Please refer to the detailed protocol when you perform a test.

Images
  • Mouse anti-Human CD3, Purified mAb - Absci

    Human peripheral blood lymphocytes analyzed with Purified CD3 mAb, followed by anti-mouse IgGs-PE

Background
 4AT3 reacts with CD3, a 20-26 kDa molecule, which is expressed on all mature T lymphocytes  (approximately 60-80% of normal human peripheral blood lymphocytes), NK-T cells and some thymocytes.  CD3 associated with the T-cell receptor a/b or g/d dimer also plays a role in T-cell activation and signal transduction during antigen recognition.
* CD3 mAb is used to count mature T cells in blood.
 
1. Ledbetter, J et al. (1981) J Exp Med 153:310-323.
2. Haynes, B et al. (1986) Leukocyte Typing II: Human T lymphocytes. New York: Sprimger-Verlag; 3-30.
3. Clevers H et al. (1988). Ann Rev Immunol 6 : 629-662.
4. Barclay, N et al. (1993). The Leucocyte Factsbook. Academic Press. San Diego.

 

 

    Please let us know if you have published research using #AB28003 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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