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Histone H3K14me1 Polyclonal AntibodyABHW034

  Catalog No. Package Size Price
ABHW034 100ul $299.00

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Description
  • Product Name Histone H3K14me1 Polyclonal Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide
  • Immunogen Description A synthetic peptide of human H3K14me1
  • Target Name Histone H3
  • Modification K14me1
  • Alternative Names H3t; H3.4; H3/g; H3FT;
  • Accession No. Gene ID: 8290 Swiss Prot: Q16695
  • SDS-PAGE MW 15kDa
  • Concentration 1.0mg/ml
  • Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage Store at -20℃ or -80℃. Avoid freeze / thaw cycles.
Application Details

WB 1:500 - 1:2000

IHC 1:50- 1:200

IF 1:20- 1:50

Images
  • Histone H3K14me1 Polyclonal Antibody - Absci

    Western blot analysis of extracts of HeLa and H3 protein, using H3K14me1 antibody.

Background

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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