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Histone H3R8me1 Polyclonal AntibodyABHW024

  Catalog No. Package Size Price
ABHW024 100ul $299.00

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Description
  • Product Name Histone H3R8me1 Polyclonal Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
  • Applications WB IHC IF ChIP
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide
  • Immunogen Description A synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3
  • Target Name Histone H3
  • Modification R8me1
  • Alternative Names HIST1H3J; H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;
  • Accession No. Gene ID: 8290 Swiss Prot: Q16695
  • SDS-PAGE MW 15kDa
  • Concentration 1.0mg/ml
  • Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage Store at -20℃ or -80℃. Avoid freeze / thaw cycles.
Application Details

WB 1:500 - 1:2000

IHC 1:50 - 1:200
IF 1:50 - 1:200

ChIP 1:50 - 1:200

Images
  • Histone H3R8me1 Polyclonal Antibody - Absci

    Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R8me1antibody.

  • Histone H3R8me1 Polyclonal Antibody - Absci

    Dot-blot analysis of all sorts of methylation peptides using H3R8me1 antibody.

  • Histone H3R8me1 Polyclonal Antibody - Absci

    Immunofluorescence analysis of 293T cell using H3R8me1 antibody. Blue: DAPI for nuclear staining.

Background

 Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

 

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
    Please let us know if you have published research using #ABHW024 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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