Histone H4R3me2a Polyclonal Antibody

Description

Product Name Histone H4R3me2a Polyclonal Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
Applications WB IF IP ChIP
Species Reactivity Hu Ms Rt
Immunogen Type Peptide
Immunogen Description A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
Target Name Histone H4
Modification R3me2a
Alternative Names H3t; H3.4; H3/g; H3FT;
Accession No. Gene ID: 8370 Swiss Prot: P62805
SDS-PAGE MW 11kDa
Concentration 1.0mg/ml
Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Store at -20℃ or -80℃. Avoid freeze / thaw cycles.

Application Details

WB 1:500 - 1:2000

IF 1:50- 1:200

IP 1:50- 1:100

ChIP 1:50- 1:200

Images

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H4R3 me2a antibody.
Dot-blot analysis of all sorts of methylation peptidesusing H4R3 me2a antibody.
Immunofluorescence analysis of 293T cell using H4R3me2a antibody. Blue: DAPI for nuclear staining.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.

Published Papers

let us know

Customer Reviews and Rankings

No comment