PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total nNOS protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.850~854 (F-N-S-V-S) derived from Human nNOS.
Target NamenNOS
Alternative NamesBNOS; Constitutive NOS; N-NOS; NC-NOS; NOS1
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 160kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from Rat and Mouse brain tissue using nNOS(Ab-852) Antibody #AB21159.
Immunofluorescence staining of methanol-fixed Hela cells using nNOS(Ab-852) Antibody #AB21159.
Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.
Liu JL et al. (2008) World J Gastroenterol. 14(20):3242-8.
Guggilam A et al. (2008) Eur J Heart Fail. 10(7):625-34
Marquina M et al. (2008) Br J Dermatol.159(1):68-76.