Histone H3(Acetyl-Lys9) Antibody

Description

Product Name Histone H3(Acetyl-Lys9) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic acetylpeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific acetylpeptide. Non-acetyl specific antibodies were removed by chromatogramphy using non-acetylpeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of Histone H3 only when acetylated at Lysine 9.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around acetylation site of Lysine 9(T-A-R(p)-K-S) derived from Human Histone H3.
Target Name Histone H3
Modification Acetyl-Lys9
Alternative Names H3/A, H3FA
Accession No. Swiss-Prot#: P68431;
NCBI Gene#: 8350;
NCBI Protein#: NP_003529.1
SDS-PAGE MW 17kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Predicted MW: 17kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Hela cells treated with TSA using Histone H3(Acetyl-Lys9) Antibody #AB12069.The lane on the left is treated with the antigen-specific peptide.

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Published Papers

let us know

Customer Reviews and Rankings

No comment