PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total NMDAR2B protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.1472~1476 (H-V-Y-E-K) derived from Human NMDAR2B.
Immunofluorescence staining of methanol-fixed Hela cells using NMDAR2B(Ab-1474) antibody #AB21158.
Background
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Babb TL et al.Epilepsy Res. 2005 Mar-Apr;64(1-2):23-30
Chazot PL. Curr Med Chem. 2004 Feb;11(3):389-96
Kakegawa W, et al.Eur J Neurosci. 2003 Feb;17(4):887-91