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HSP70 Monoclonal AntibodyAB27152

  Catalog No. Package Size Price
AB27152 100ul $298.00

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Description
  • Product Name HSP70 Monoclonal Antibody
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity-chromatography
  • Applications WB IHC ICC
  • Species Reactivity Hu
  • Specificity HSP70 antibody detects endogenous levels of total HSP70
  • Immunogen Type Recombinant protein
  • Immunogen Description Purified recombinant fragment of human HSP70 expressed in E. Coli
  • Target Name HSP70
  • Alternative Names RY; APG-2; hsp70; hsp70RY; HS24/P52; MGC131852; HSPA4
  • Accession No. Swiss-Prot#: P08107
  • SDS-PAGE MW 70KD
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C/1 year
Application Details

 ELISA: 1:10000
   WB: 1:500 - 1:2000
 IHC: 1:200 - 1:1000
 ICC: 1:200 - 1:1000

Images
  • HSP70 Monoclonal Antibody - Absci

    Western blot analysis using HSP70 mouse mAb against Hela (1) cell lysate.

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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