FKHR(Ab-256) Antibody

Description

Product Name FKHR(Ab-256) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Human Mouse Rat
Specificity The antibody detects endogenous level of total FKHR protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.254~258 (A-A-S-M-D) derived from Human FKHR/FOXO1A.
Target Name FKHR
Alternative Names FOXO1
Accession No. Swiss-Prot: Q12778
NCBI Protein: NP_002006.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 78-82 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from Hela cells using FKHR(Ab-256) Antibody #AB21138.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR(Ab-256) Antibody #AB21138(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using FKHR(Ab-256) Antibody #AB21138.

Background

FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

Gan L, et al. (2005) J Neurochem; 93(5): 1209-19.
Smith WW, et al. (2005) J Cell Biol; 169(2): 331-9.
Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77.
Horn S, et al. (2004) Leukemia; 18(11): 1839-49.
Zhao X, et al. (2004) Biochem J 4; 378(Pt 3): 839-49.

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