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TERT AntibodyAB31222

  Catalog No. Package Size Price
AB31222-1 50ul $189.00
AB31222-2 100ul $279.00

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Description
  • Product Name TERT Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Applications E WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of total TERT protein.
  • Immunogen Type Peptide
  • Immunogen Description Synthetic peptide corresponding to a region derived from 1120-1132 amino acids of human telomerase reverse transcriptase
  • Target Name TERT
  • Alternative Names telomerase reverse transcriptase, TP2, TRT, EST2, TCS1, hTRT, DKCA2, DKCB4, hEST2, PFBMFT1
  • Accession No. Genbank No.: NP_937983
  • Concentration 1.2mg/ml
  • Formulation Supplied at 1.2mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
  • Storage Store at -20˚C/1 year
Application Details

Predicted MW: 120kd
ELISA: 1:1000-1:5000
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:50-1:200

Images
  • TERT Antibody - Absci

    Gel: 8%SDS-PAGE
    Lane1:Human mucinous soft sarcoma tissue lysate
    Lane2:Human gliomas tissue lysate
    Lane3: HT-29 cell lysate
    Lysates: 40 ug per lane
    Primary antibody: 1/600 dilution
    Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
    Exposure time: 40 seconds

  • TERT Antibody - Absci

    The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using AB31222 (TERT Antibody) at dilution 1/30, on the right is treated with the synthetic peptide.

  • TERT Antibody - Absci

    All lanes : TERT Antibody (AB31222) at 1/500 dilution Lane 1 : Human mucinous soft sarcoma tissue lysate Lysates/proteins at 40 µg per lane. Predicted band size : 120 kDa Observed band size : 120 kDa

  • TERT Antibody - Absci

    AB31222 at 1/100 staining Human Ovarian cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in formaldehyde buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4°C. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

  • TERT Antibody - Absci

    AB31222 at 1/100 staining Human Thyroid cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in formaldehyde buffer was performed. The tissue was then blocked and incubated with the antibody for one night at 4°C. A Goat Anti-Rabbit IgG H&L (HRP) at 1/200 was used as secondary.

Background

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.

    Please let us know if you have published research using #AB31222 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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