ASK1(Ab-966) Antibody

Description

Product Name ASK1(Ab-966) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total ASK1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.964~968 (S-I-S-L-P) derived from Human ASK1.
Target Name ASK1
Alternative Names ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
Accession No. Swiss-Prot: Q99683
NCBI Protein: NP_005914.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from 293 and Hela cells using ASK1(Ab-966) Antibody #AB21134.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-966) Antibody #AB21134(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-966) Antibody #AB21134.

Background

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044.
Fujii K, et al. (2004) Oncogene. 23(29):5099-5104.
Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449.
Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.

Published Papers

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