AMPKα1(Ab-496)Antibody

Description

Product Name AMPKα1(Ab-496)Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous level of total AMPKa1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.494~498 (S-G-S-V-S) derived from Human AMPKα1.
Target Name AMPKa1
Alternative Names AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1
Accession No. Swiss-Prot: Q13131/P54646
NCBI Protein: NP_006242.5
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from 293 cells using AMPKα1 (Ab-496)Antibody #AB21130.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Ab-496)Antibody #AB21130 (left) or the same antibody preincubated with blocking peptide (right).

Background

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Published Papers

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