PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total PKD/PKCm protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 908~912 (R-V-S-I-L) derived from Human PKD/PKCm.
Target NamePKD/PKCm
Alternative NamesnPKC-D1; Protein kinase D; Protein kinase C mu type; nPKC-mu;
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 115kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from Hela, 293 and 3T3 cells using PKD/PKCm(Ab-910) Antibody #AB21128.
Background
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Matthews SA, et al. J Biol Chem 1999 Sep; 274(37): 26543-26549
Brandlin I, et al. J Biol Chem 2002 Feb; 277(8): 6490-6496
Storz P, et al. Mol Pharmacol 2004 Oct; 66(4): 870-879