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MKK3(Ab-189) AntibodyAB21116

  Catalog No. Package Size Price
AB21116-1 50ul $189.00
AB21116-2 100ul $279.00
AB21116-4 25ul $119.00

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Description
  • Product Name MKK3(Ab-189) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous level of total MKK3 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.
  • Target Name MKK3
  • Alternative Names MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3
  • Accession No. Swiss-Prot: P46734
    NCBI Protein: NP_002747.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • MKK3(Ab-189) Antibody - Absci

    Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody #AB21116.

  • MKK3(Ab-189) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Ab-189) Antibody #AB21116(left) or the same antibody preincubated with blocking peptide(right).

  • MKK3(Ab-189) Antibody - Absci

    Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Ab-189) Antibody #AB21116.

Background

MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

    Please let us know if you have published research using #AB21116 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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