Welcome     Login   Register   Message Board   
Your Position: Home > Cell Signal Transduction > Chk1(Ab-317) Antibody

Chk1(Ab-317) AntibodyAB21114

  Catalog No. Package Size Price
AB21114-1 50ul $189.00
AB21114-2 100ul $279.00
AB21114-4 25ul $119.00

Quantity:  Total:   
 

Review:comment rank 5 pdf

Availability: promotionYes

add promotion
Description
  • Product Name Chk1(Ab-317) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of total Chk1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.
  • Target Name Chk1
  • Alternative Names CHEK1
  • Accession No. Swiss-Prot: O14757
    NCBI Protein: NP_001107593.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • Chk1(Ab-317) Antibody - Absci

    Western blot analysis of extracts from MDA231,Hela and 293 cells using Chk1(Ab-317) Antibody #AB21114.

  • Chk1(Ab-317) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1(Ab-317) Antibody #AB21114(left) or the same antibody preincubated with blocking peptide(right).

Background

Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry

Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18.
Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.
Rocha S, et al. (2005) EMBO J.
Clarke CA, et al. (2005) Biochem J.
Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.

    Please let us know if you have published research using #AB21114 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    E-mail:
    Ratings:
    Content:
    Verification code: captcha
     
    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


    © 2017, AbSci All Rights Reserved. E-mail: info@abscitech.com
    南京川博生物技术有限公司版权所有
    苏B1-20150380 苏ICP备15009006号-1