4E-BP1(Phospho-Thr37) Antibody

Description

Product Name 4E-BP1(Phospho-Thr37) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of 4E-BP1 only when phosphorylated at threonine 37.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 37(S-T-T(p)-P-G) derived from Human 4E-BP1.
Target Name 4E-BP1
Modification Phospho-Thr37
Alternative Names BP-1; 4EBP1; 4E-BP1; PHAS-I;
Accession No. Swiss-Prot: Q13541; NCBI Gene ID: 1978; NCBI mRNA: NM_004095.3; NCBI Protein: NP_004086.1
SDS-PAGE MW 18KD
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Predicted MW: 18kd

Western blotting : 1:500~1:1000

Immunohistochemistry: 1:50~1:100

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr37) antibody (#11222).
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-37) antibody (#21215, Lane 1 and 2) and 4E-BP1 (phospho-Thr37) antibody (#11222, Lane 3 and 4).

Background

Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Published Papers

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