PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Tau protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.212~216 (T-P-S-L-P) derived from Human Tau.
Target NameTau
Alternative NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
Immunofluorescence staining of methanol-fixed Hela cells using Tau(Ab-214) Antibody #AB21098.
Background
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
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