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NFκB-p65(Phospho-Ser276) AntibodyAB11011

  Catalog No. Package Size Price
AB11011-1 50ul $189.00
AB11011-2 100ul $279.00
AB11011-4 25ul $119.00

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Description
  • Product Name NFκB-p65(Phospho-Ser276) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of NFκB-p65 only when phosphorylated at serine 276.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NFκB-p65.
  • Target Name NFκB-p65
  • Modification Phospho-Ser276
  • Alternative Names p65, NFKB3
  • Accession No. Swiss-Prot#:Q04206 NCBI Gene#:5970 NCBI Protein#:NP_001138610.1
  • SDS-PAGE MW 65KD
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C/1 year
Application Details

Western blotting: 1:500-1:1000 

Immunofluorescence: 1:100-1:200 
Immunohistochemistry: 1:50-1:100 
 

Images
  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser276) Antibody #AB11011.

  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Western blot analysis of extract from Hela cells using NF-κB p65 (phospho-Ser276) antibody #AB11011. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non- phospho peptide blocking peptides #BP61011. Lane 3: The antibody is preincubated with phospho peptide blocking peptides #BP51011.

  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody #AB11011 (left) or the same antibody preincubated with blocking peptide #BP51011 (right).

  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody #AB11011.

  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody #AB11011.

  • NFκB-p65(Phospho-Ser276) Antibody - Absci

    Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFκB-p65 (Phospho-Ser276) Antibody #AB11011.

Background

 NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

    Please let us know if you have published research using #AB11011 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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