PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p53 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 44~48 (M-L-S-P-D) derived from Human p53.
Target Namep53
Alternative NamesTumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from HT29 cells using p53(Ab-46) Antibody #AB21090.
Background
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
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Di Stefano V, et al. (2005) Oncogene. 24(35):5431-5442.
Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959.
Wang L, et al. (2005) Oncogene. 24(18): 3020-3027.