PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total VEGFR2 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.1173~1177 (K-D-Y-I-V) derived from Human VEGFR2.
Target NameVEGFR2
Alternative NamesFLK1; KDR; VGFR2; VGR2; kinase insert domain receptor
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Ab-1175) Antibody #AB21077(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Ab-1175) Antibody #AB21077.
Background
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions
Lamalice L, et al. (2004). Oncogene.23(2): 434-445.
Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.