Twist1 Antibody

Description

Product Name Twist1 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous levels of total Twist1 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.18~22(S-N-S-E-E) derived from Human Twist1
Target Name Twist1
Alternative Names SCS; ACS3; CRS1; BPES2; BPES3
Accession No. Swiss-Prot: Q15672
NCBI Protein: NP_000465.1
Calculated MW 28kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Western blotting 1:500-1:1000
Immunohistochemistry 1:100-1:500

Images

Western blot analysis of extracts from JK, Raji and HL-60 cells using Twist1 Antibody #AB21642.
Twist1 Antibody staining Twist in Mouse pancreatic cancer tissue sections by Immunohistochemistry

Background

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity."Sosic D., Richardson J.A., Yu K., Ornitz D.M., Olson E.N.Cell 112:169-180(2003)
"Mutations of the TWIST gene in the Saethre-Chotzen syndrome."el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46(1997)

Published Papers

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