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Twist2 AntibodyAB21669

  Catalog No. Package Size Price
AB21669-1 50ul $189.00
AB21669-2 100ul $279.00
AB21669-4 25ul $119.00

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Description
  • Product Name Twist2 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of total Twist2 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.26~30(P-K-R-F-G) derived from Human Twist2.
  • Target Name Twist2
  • Alternative Names DERMO1; MGC117334; bHLHa39
  • Accession No. Swiss-Prot: Q8WVJ9
    NCBI Protein: NP_476527.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Western blotting      1:100-1:500
Immunohistochemistry  1:100-1:500

Images
  • Twist2 Antibody - Absci

    Western blot analysis of extracts from A549, Hela and 3T3 cells using Twist2 Antibody #AB21669.

Background

Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism. Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors.

Lee M.S., Lowe G., Flanagan S., Kuchler K., Glackin C.A.Bone 27:591-602(2000)

    Please let us know if you have published research using #AB21669 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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