PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa. 1090~1094 (P-E-Y-I-N) derived from Human EGFR.
Target NameEGFR
Alternative Names Receptor tyrosine-protein kinase ErbB-1
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from HepG2, 293 and Hela cells using EGFR(Ab-1092) Antibody #AB21074.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1092) Antibody #AB21074(left) or the same antibody preincubated with blocking peptide(right).
Background
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
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