HER2(Ab-1221/1222) Antibody

Description

Product Name HER2(Ab-1221/1222) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC IF
Species Reactivity Hu
Specificity The antibody detects endogenous level of total HER2 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.1219~1223/1220~1224 (N-L-Y-Y -W) derived from Human HER2.
Target Name HER2
Alternative Names C-erbB-2; ErbB2;
Accession No. Swiss-Prot: P04626
NCBI Protein: NP_001005862.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from SKOV3 and MDA cells using HER2(Ab-1221/1222) Antibody #AB21071.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1221/1222) Antibody #AB21071(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using HER2(Ab-1221/1222) Antibody #AB21071.

Background

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.
Ouyang X,et al. (1998)Carcinogenesis; 19(11): 2013-9.
Ouyang X,et al. (1998) Exp Cell Res; 241(2): 467-75.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

Published Papers

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