PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Estrogen Receptor-a protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.102~106 (S-V-S-P-S) derived from Human Estrogen Receptor-a.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 66kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from MCF-7
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Ab-104) Antibody #AB21065(left) or the same antibody preincubated with blocking peptide(right).
Background
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
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Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
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Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.