PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total JunB protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.257~261 (P-V-S-P-I) derived from Human JunB.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from Hela cells using JunB(Ab-259) Antibody #AB21027 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Ab-259) Antibody #AB21027(left) or the same antibody preincubated with blocking peptide(right).
Background
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.