PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total JunB protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.77~81 (G-A-S-L-K) derived from Human JunB.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Ab-79) Antibody #AB21026(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using JunB(Ab-79) Antibody #AB21026.
Background
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Narayanan K, et al. (2004) J Biol Chem. 279(43): 44294-442302.