PurificationAntibodies were produced by immunizing goat with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1197 (A-E-Y(p)-L-R) derived from Human EGFR.
Target NameEGFR
ModificationPhospho-Tyr1197
Alternative Names Receptor tyrosine-protein kinase ErbB-1; ERBB1; kinase EGFR
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from PMA-treated HUVEC cells or EGF-treated MDA cells using EGFR(phospho-Tyr1197) goat polyclonal antibody #AB11551.
Background
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Corbalan-Garcia S, et al. (1996) Mol Cell Biolt; 16(10): 5674-5682
Kanner SB, et al. (1991) Mol Cell Biol; 11(2): 713-720
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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