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CDK6(phospho-Tyr13) AntibodyAB11542

  Catalog No. Package Size Price
AB11542-1 50ul $189.00
AB11542-2 100ul $279.00
AB11542-4 25ul $119.00

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Description
  • Product Name CDK6(phospho-Tyr13) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 13.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of tyrosine 13 (Q-Q-Y(p)-E-C) derived from Human CDK6.
  • Target Name CDK6
  • Modification Phospho-Tyr13
  • Alternative Names Serine/threonine-protein kinase PLSTIRE
  • Accession No. Swiss-Prot: Q00534
    NCBI Protein: NP_001138778.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 36kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images
  • CDK6(phospho-Tyr13) Antibody - Absci

    Western blot analysis of extracts from 293 cells untreated or treated with HU using CDK6(phospho-Tyr13) Antibody #AB11542.

  • CDK6(phospho-Tyr13) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK6(Phospho-Tyr13) Antibody #AB11542(left) or the same antibody preincubated with blocking peptide(right).

  • CDK6(phospho-Tyr13) Antibody - Absci

    Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr13) Antibody #AB11542.

  • CDK6(phospho-Tyr13) Antibody - Absci

    Western blot analysis of extracts from HUVEC cells, treated with calf intestinal phosphatase (CIP), using CDK6 (phospho-Tyr13) Antibody #AB11542.

Background

Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.

    Please let us know if you have published research using #AB11542 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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