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HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) AntibodyAB11517

  Catalog No. Package Size Price
AB11517-1 50ul $189.00
AB11517-2 100ul $279.00
AB11517-4 25ul $119.00

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Description
  • Product Name HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
  • Target Name HDAC4/HDAC5/HDAC9
  • Modification Phospho-Ser246/259/220
  • Alternative Names HD4/HD5/HD9
  • Accession No. Swiss-Prot: P56524 Q9UQL6 Q9UKV0
    NCBI Protein: NP_006028.2/NP_001015053.1 /NP_055522.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 140 124 111 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody - Absci

    Western blot analysis of extracts from 293 cells and Hela cells treated with serum using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Antibody #AB11517.

  • HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9(Phospho-Ser246/259/220) Antibody #AB11517(left) or the same antibody preincubated with blocking peptide(right).

  • HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody - Absci

    Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC4/HDAC5/HDAC9(phospho-Ser246/259/220) Antibody #AB11517.

Background

Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.

Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
Vigushin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218.
Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

    Please let us know if you have published research using #AB11517 so that we can cite your reference.
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    Protocol
    Browsing historyclear
    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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