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eIF4G (phospho-Ser1231) AntibodyAB11514

  Catalog No. Package Size Price
AB11514-1 50ul $189.00
AB11514-2 100ul $279.00
AB11514-4 25ul $119.00

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Description
  • Product Name eIF4G (phospho-Ser1231) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of eIF4G only when phosphorylated at serine 1231.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 1231 (P-V-S(p)-P-L) derived from Human eIF4G.
  • Target Name eIF4G
  • Modification Phospho-Ser1231
  • Accession No. Swiss-Prot: Q04637
    NCBI Protein: NP_004944.2
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images
  • eIF4G (phospho-Ser1231) Antibody - Absci

    Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using eIF4G(phospho-Ser1231) Antibody #AB11514.

  • eIF4G (phospho-Ser1231) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G(Phospho-Ser1231) Antibody #AB11514(left) or the same antibody preincubated with blocking peptide(right).

Background

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.

    Please let us know if you have published research using #AB11514 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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