PKCd(Phospho-Ser645) Antibody

Description

Product Name PKCd(Phospho-Ser645) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Rt
Specificity The antibody detects endogenous level of PKCd only when phosphorylated at serine 645.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 645 (R-L-S(p)-Y-S) derived from Human PKCd.
Target Name PKCd
Modification Phospho-Ser645
Alternative Names KPCD; PKC-delta; PRKCD; kinase PKC-delta; nPKC-delta
Accession No. Swiss-Prot: Q05655
NCBI Protein: NP_006245.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 78kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCd(Phospho-Ser645) Antibody #AB11296.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCd(Phospho-Ser645) Antibody #AB11296(left) or the same antibody preincubated with blocking peptide(right).

Background

This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.

Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab ; 285: E1081 - E1088.
Ling Zhang, et,al. (2004) J. Biol. Chem ; 279: 28315 - 28319.
Kristof Van Kolen et,al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et,al. (2002) J. Cell Biol ; 157: 253.

Published Papers

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