PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level ofa-Synuclein only when phosphorylated at tyrosine 136.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 136 (Q-D-Y(p)-E-P) derived from Human a-Synuclein.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from mouse brain tissue using a-Synuclein(Phospho-Tyr136) Antibody #AB11286 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Phospho-Tyr136) Antibody #AB11286.
Background
May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.