PurificationThe antibody was purified by immunogen affinity chromatography.
ApplicationsWB IHC
Species ReactivityHu,Ms,Rt
SpecificityRecognizes endogenous levels of NFYB protein.
Immunogen DescriptionKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NFYB.
Target NameNFYB
Alternative NamesHAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB
Accession No.Swiss-Prot#:P25208 NCBI Gene ID:4801
Calculated MW32KD
Concentration1 mg/ml
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting:1:500 - 1:1000
Immunohistochemistry:1:50 - 1:100
Images
Western blot analysis of MRPL22 expression in Hela, HepG2 whole cell lysates.
Immunohistochemical analysis of NFYB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.