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AMPKα1/2 (phospho Thr183/172) Polyclonal AntibodyAB14099

  Catalog No. Package Size Price
14099-1 50ul $189.00
14099-2 100ul $279.00

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Description
  • Product Name AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody
  • Host Species Rabbit
  • Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Applications IF/ICC,WB,IHC-p,ELISA
  • Species Reactivity Human,Mouse,Rat,Monkey,Pig
  • Specificity Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.
  • Immunogen Description The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
  • Alternative Names PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
  • Accession No. Swiss Prot:Q13131/P54646
    GeneID:5562/5563
  • SDS-PAGE MW 63
  • Concentration 1 mg/ml
  • Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Storage -20˚C/1
Application Details

IF: 1:50-200 Western Blot: 1/500 - 1/2000. 
Immunohistochemistry: 1/100 - 1/300. 
ELISA: 1/40000. Not yet tested in other applications.

Background

protein kinase AMP-activated catalytic subunit alpha 1(PRKAA1) Homo sapiens The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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