KLF1/5/7 Antibody

Description

Product Name KLF1/5/7 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification The antibody was purified by immunogen affinity chromatography.
Applications WB IF
Species Reactivity Hu Ms Rt
Specificity Recognizes endogenous levels of KLF1/5/7 protein.
Immunogen Description KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human KLF1/5/7.
Target Name KLF1; KLF5; KLF7
Alternative Names KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; KLF7; UKLF; Krueppel-like factor 7; Ubiquitous krueppel-like factor
Accession No. Swiss-Prot#:Q13351; Q13887; O75840
NCBI Gene ID:10661; 688; 8609
Calculated MW 38KD
Concentration 1 mg/ml
Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Western blotting:1:500 - 1:1000
Immunofluorescence:1:100 - 1:300

Images

Western blot analysis of GALR2 expression in A431 whole cell lysates.
Immunofluorescent analysis of KLF1/5/7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.