SpecificityPhospho-MOB1A (S38) Antibody detects endogenous levels of MOB1A only when phosphorylated at S38
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human MOB1A (Phospho-Ser38)
Alternative NamesMATS 2 antibody
MATS2 antibody
MGC33910 antibody
Mob 1A antibody
Mob 1B antibody
MOB 4A antibody
MOB kinase activator 1B antibody
Mob1 homolog 1A antibody
MOB1 Mps One Binder homolog B antibody
MOB1 Mps one binder kinase activator like 1A antibody
Mob1A antibody
Mob1B antibody
MOBKL 1A antibody
MOBKL1A antibody
MOL1A_HUMAN antibody
Mps one binder kinase activator like 1A antibody
Mps one binder kinase activator-like 1A antibody
Protein Mob4A antibody
Accession No.Swiss-Prot#:Q7L9L4 NCBI Gene ID92597
Calculated MW25
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis MOB1A (Phospho-Ser38) using EGF treated 293 whole cell lysates
Product Description
MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1α and MOB1β, that are encoded by two different genes but have 96.3% identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1 and 2 and NDR1 and 2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues by MST1 and,or MST2 (5,10).
Phosphorylation at Thr12 by MST1,2 stabilizes MOB1, enhancing its binding and regulation of LATS1 (5). The resultant increase in LATS1 kinase activity promotes inhibitory phosphorylation of the transcriptional co-activators YAP and TAZ (11,12), leading to changes in the expression of genes involved in cell cycle progression (13).
Yes
