SpecificityPhospho-FoxO3a (S574) Antibody detects endogenous levels of FoxO3a only when phosphorylated at S574
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human FoxO3a (Phospho-Ser574)
Alternative NamesAF6q21 antibody
AF6q21 protein antibody
DKFZp781A0677 antibody
FKHR2 antibody
FKHRL 1 antibody
FKHRL1 antibody
FKHRL1P2 antibody
Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody
Forkhead box O3 antibody
Forkhead box O3A antibody
Forkhead box protein O3 antibody
Forkhead box protein O3A antibody
Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody
Forkhead homolog (rhabdomyosarcoma) like 1 antibody
Forkhead in rhabdomyosarcoma like 1 antibody
Forkhead in rhabdomyosarcoma-like 1 antibody
FOX O3A antibody
FOXO2 antibody
foxo3 antibody
FOXO3_HUMAN antibody
FOXO3A antibody
MGC12739 antibody
MGC31925 antibody
Accession No.Swiss-Prot#:O43524 NCBI Gene ID2309
Calculated MW97
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis FoxO3a (Phospho-Ser574) using TNF-a treated 293 whole cell lysates
Product Description
The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).
p38 phosphorlyates FoxO3a at Ser7 and promotes its nuclear localization (10).