SpecificityPhospho-ULK1(S757) Antibody detects endogenous levels of ULK1 only when phosphorylated at S757
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human ULK1(Phospho-Ser757)
Alternative NamesATG 1 antibody
ATG1 antibody
ATG1 autophagy related 1 homolog antibody
ATG1A antibody
Autophagy related protein 1 homolog antibody
Autophagy-related protein 1 homolog antibody
FLJ38455 antibody
FLJ46475 antibody
hATG1 antibody
KIAA0722 antibody
Serine threonine protein kinase ULK1 antibody
Serine threonine protein kinase Unc51.1 antibody
Serine threonine-protein kinase ULK1 antibody
ULK 1 antibody
ULK1 antibody
ULK1_HUMAN antibody
Unc 51 (C. elegans) like kinase 1 antibody
UNC 51 antibody
Unc 51 like kinase 1 antibody
Unc-51 like kinase 1 (C. elegans) antibody
Unc-51-like kinase 1 antibody
UNC51 antibody
UNC51 C. elegans homolog of antibody
Unc51.1 antibody
Accession No.Swiss-Prot#:O75385 NCBI Gene ID8408
Calculated MW140-150
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis ULK1(Phospho-Ser757) using A431 whole cell lysates
Product Description
Two related serine,threonine kinases, UNC-51-like kinase -1 and -2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline,serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors such as NGF (5). Yeast two-hybrid studies found ULK1,2 associated with modulators of the endocytic pathway, SynGap, and syntenin (6). Structural similarity of ULK1,2 has also been recognized with the yeast autophagy protein Atg1,Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1,ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).