PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of synapsin only when phosphorylated at serine 9.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of serine 9 (R-L-S(p)-D-S) derived from Human SYN1/synapsin.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 77kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from Mouse Brain tissue using synapsin(Phospho-Ser9) Antibody #AB11267.
Immunofluorescence staining of methanol-fixed Hela cells using synapsin(Phospho-Ser9) Antibody #AB11267.
Background
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level