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Merlin(Phospho-Ser518) AntibodyAB11266

  Catalog No. Package Size Price
AB11266-1 50ul $189.00
AB11266-2 100ul $279.00
AB11266-4 25ul $119.00

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Description
  • Product Name Merlin(Phospho-Ser518) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications WB IF
  • Species Reactivity Hu Ms Rt
  • Specificity The antibody detects endogenous level of Merlin only when phosphorylated at serine 518.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin.
  • Target Name Merlin
  • Modification Phospho-Ser518
  • Alternative Names MERL; NF2; Neurofibromin 2; SCH; Schwannomerlin
  • Accession No. Swiss-Prot: P35240
    NCBI Protein: NP_000259.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 69kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images
  • Merlin(Phospho-Ser518) Antibody - Absci

    Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) Antibody #AB11266.

  • Merlin(Phospho-Ser518) Antibody - Absci

    Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) Antibody #AB11266.

  • Merlin(Phospho-Ser518) Antibody - Absci

    Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody #AB11266.

Background

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.

Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394.
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715.
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545.
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.

    Please let us know if you have published research using #AB11266 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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