PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThis antibody detects endogenous levels of Thioredoxin
Immunogen Typeprotein
Immunogen DescriptionSynthesized peptide derived from human Thioredoxin. at AA range: 52-101
Target NameThioredoxin
Alternative NamesThioredoxin (Trx) (ATL-derived factor) (ADF) (Surface-associated sulphydryl protein) (SASP)
Accession No.Swiss-Prot:P10599 NCBI Gene ID:7295
Calculated MW12kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20˚C
Application Details
Western blotting: 1:500-1:2000
Images
Western Blot analysis of 1,mouse-liver 2,hela cells using primary antibody diluted at 1:1000(4˚C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25˚C, 1 hour)